human haptoglobin elisa kit  (Assaypro)

Journal: Biochemistry and biophysics reports

Article Title: Transcription factor SP1 regulates haptoglobin fucosylation via induction of GDP-fucose transporter 1 in the hepatoma cell line HepG2.

doi: 10.1016/j.bbrep.2022.101372

Figure Lengend Snippet: Fig. 1. Changes in Fuc-Hp and candidate regula tory gene expression upon IL-6 stimulation. A) Fuc-Hp levels in the culture media of IL- 6–stimulated HepG2 cells were quantified by AAL ELISA. Data are presented as the average ± S.D. (N = 3 for each condition). *p < 0.05. B) Relative expres sion of HP and fucosylation regulatory genes in HepG2 cells stimulated by IL-6. Data are normalized to unstimulated (0 ng/ml IL-6) cells and presented as the average ± S.D. (N = 3 for each condition). *p < 0.05.

Article Snippet: To determine the total Hp concentration, the AssayMaxTM Human Haptoglobin ELISA kit (Assaypro, St. Charles, MO) was used according to the manufacturer’s protocol with control biotinylated human Hp protein diluted 10-fold.

Techniques: Gene Expression, Enzyme-linked Immunosorbent Assay

Journal: International journal of cancer

Article Title: Serum haptoglobin as a novel molecular biomarker predicting colorectal cancer hepatic metastasis.

doi: 10.1002/ijc.29993

Figure Lengend Snippet: Figure 1. Elevated HP levels in colorectal cancer patients. (a) Serum levels of HP in healthy controls and colorectal cancer patients. (b) The serum levels of HP in healthy controls (n 5 152) and colorectal cancer patients [M0, M1(S), and M1(H), n 5 250] were measured by ELISA. (c) Kaplan–Meier survival curve showed correlation between HP serum level and overall survival. (*, p<0.05) [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Article Snippet: Measurement of HP level in human blood samples A commercially available ELISA kit Human Haptoglobin ELISA Kit(Assaypro EH1003-1)was used to measure HP level.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Impact of Virgin Olive Oil and Phenol-Enriched Virgin Olive Oils on the HDL Proteome in Hypercholesterolemic Subjects: A Double Blind, Randomized, Controlled, Cross-Over Clinical Trial (VOHF Study)

doi: 10.1371/journal.pone.0129160

Figure Lengend Snippet: Expression levels of Apo A-I and A-II were measured by immunturbidimetry assays and clusterin and haptoglobin by ELISA assays in 10 HDL pools.

Article Snippet: The quantification of haptoglobin and clusterin was performed using two commercial ELISA assays following manufacter’s instructions; the AssayMax Haptoglobin ELISA kit (AssayPro, MO, USA) and the Human Clusterin ELISA Kit (RayBiotech, GA, USA), respectively.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Open Access Macedonian Journal of Medical Sciences

Article Title: Clinical Relevance of Transforming Growth Factor-β1, Interleukin-6 and Haptoglobin for Prediction of Obesity Complications in Prepubertal Egyptian Children

doi: 10.3889/oamjms.2015.017

Figure Lengend Snippet: The anthropometric measures, and pro-inflammatory cytokines of obese group and overweight group versus control group.

Article Snippet: Serum haptoglobin was assayed by Sandwich enzyme linked immunosorbent assay (ELISA) as the method using a Human haptoglobin assay kit purchased from Assay Pro Co. (USA) according to Van Vlierberghe et al., [ ].

Techniques: Control

Journal: Open Access Macedonian Journal of Medical Sciences

Article Title: Clinical Relevance of Transforming Growth Factor-β1, Interleukin-6 and Haptoglobin for Prediction of Obesity Complications in Prepubertal Egyptian Children

doi: 10.3889/oamjms.2015.017

Figure Lengend Snippet: Correlations between anthropometric measurements and pro-inflammatory cytokines in obese / overweight group.

Article Snippet: Serum haptoglobin was assayed by Sandwich enzyme linked immunosorbent assay (ELISA) as the method using a Human haptoglobin assay kit purchased from Assay Pro Co. (USA) according to Van Vlierberghe et al., [ ].

Techniques:

Journal: Open Access Macedonian Journal of Medical Sciences

Article Title: Clinical Relevance of Transforming Growth Factor-β1, Interleukin-6 and Haptoglobin for Prediction of Obesity Complications in Prepubertal Egyptian Children

doi: 10.3889/oamjms.2015.017

Figure Lengend Snippet: Correlations between serum level of IL-6 and body mass index, serum levels of haptoglobin, and TGF- β1 in overweight group.

Article Snippet: Serum haptoglobin was assayed by Sandwich enzyme linked immunosorbent assay (ELISA) as the method using a Human haptoglobin assay kit purchased from Assay Pro Co. (USA) according to Van Vlierberghe et al., [ ].

Techniques:

Journal: Open Access Macedonian Journal of Medical Sciences

Article Title: Clinical Relevance of Transforming Growth Factor-β1, Interleukin-6 and Haptoglobin for Prediction of Obesity Complications in Prepubertal Egyptian Children

doi: 10.3889/oamjms.2015.017

Figure Lengend Snippet: Linear logistic regression between body mass index and serum pro-inflammatory cytokines in obese / overweight group.

Article Snippet: Serum haptoglobin was assayed by Sandwich enzyme linked immunosorbent assay (ELISA) as the method using a Human haptoglobin assay kit purchased from Assay Pro Co. (USA) according to Van Vlierberghe et al., [ ].

Techniques: